Hepatitis B Virus cccDNA Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0731-ZJF)

The kit uses SYBR Green real-time PCR to detect Hepatitis B Virus cccDNA by amplifying its conserved gene segments. The specific primers and optimized reaction mix enable efficient amplification and signal generation. Fluorescence is captured in real time by the PCR instrument, facilitating sensitive and accurate analysis of the target nucleic acid.

Kit Components:
Hepatitis B Virus cccDNA Amplification Mix: 50 µL × 1 tube
Hepatitis B Virus cccDNA Reaction Mix: 950 µL × 1 tube
Hepatitis B Virus cccDNA Positive Control: 50 µL × 1 tube
Hepatitis B Virus cccDNA Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Designed for immediate use with user-provided DNA.
Selective primers crafted from highly conserved genetic regions.
Fine-tuned probe and primer chemistry enhances detection power.
Includes control sample for clear interpretation.
Sealed fluorescence PCR tube reduces contamination possibilities.
For investigational use in laboratories.

STEM-SLSR-0729-ZJF
STEM-SLSR-0730-ZJF
STEM-SLSR-0732-ZJF
STEM-SLSR-0733-ZJF