Tilletia Controversa (Dwarf Bunt of Wheat) Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0046-ZJF)

This real-time PCR detection kit is based on SYBR Green fluorescence chemistry and is intended for the analysis of Tilletia Controversa (Dwarf Bunt of Wheat) nucleic acids. Primers targeting conserved regions enable specific amplification. During the reaction, fluorescent signals are emitted and continuously tracked by the system, providing reliable, real-time quantification and identification of the target sequence.

Kit Components:
Tilletia Controversa (Dwarf Bunt of Wheat) Amplification Mix: 50 µL × 1 tube
Tilletia Controversa (Dwarf Bunt of Wheat) Reaction Mix: 950 µL × 1 tube
Tilletia Controversa (Dwarf Bunt of Wheat) Positive Control: 50 µL × 1 tube
Tilletia Controversa (Dwarf Bunt of Wheat) Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Ready-to-use kit requiring only user-supplied DNA template.
High specificity: Primers are designed based on highly conserved regions of Tilletia controversa (the pathogen of wheat dwarf bunt), ensuring no amplification of other bacterial DNA.
Optimized primers and amplification system provide 100-fold greater sensitivity compared to conventional PCR.
Includes non-infectious PCR positive control to help distinguish false-negative results.
Closed one-tube fluorescence PCR operation minimizes the risk of environmental contamination.
For research use only; kit provides sufficient reagents for 50 PCR reactions with a 2 μL reaction volume.

STEM-SLSR-0044-ZJF
STEM-SLSR-0045-ZJF
STEM-SLSR-0047-ZJF
STEM-SLSR-0048-ZJF