Sheep-Derived Component Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0035-ZJF)

Designed for the detection of Sheep-Derived Component, this kit employs SYBR Green-based real-time PCR. It uses highly specific primers to amplify conserved gene regions of the target sequence. The real-time detection system monitors fluorescence generated during amplification, allowing for accurate qualitative and quantitative evaluation of Sheep-Derived Component in various biological or food samples.

Kit Components:
Sheep-Derived Component Amplification Mix: 50 µL × 1 tube
Sheep-Derived Component Reaction Mix: 950 µL × 1 tube
Sheep-Derived Component Positive Control: 50 µL × 1 tube
Sheep-Derived Component Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Ready-to-use kit requiring only user-provided DNA template.
High specificity: Primers are designed based on highly conserved regions of ovine (sheep-derived) DNA and do not amplify non-ovine components.
Optimized primers and amplification system offer sensitivity 100 times higher than conventional PCR.
Includes non-infectious PCR positive control to help distinguish false-negative results.
One-tube fluorescence PCR workflow minimizes the risk of environmental contamination.
For research use only; the kit provides sufficient reagents for 50 PCR reactions using a 2 μL system.

STEM-SLSR-0033-ZJF
STEM-SLSR-0034-ZJF
STEM-SLSR-0036-ZJF
STEM-SLSR-0037-ZJF