Porcine Torque Teno Virus Type 2 (Porcine Circovirus Type 2) qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0022-ZJF)

This kit utilizes PCR technology, designed with specific primers targeting the highly conserved genomic region of the Porcine Torque Teno Virus Type 1 (TTSuV-1). PCR technology is used to amplify and detect the nucleic acids of TTSuV-1 in vitro. In the reaction system, when the TTSuV-1 nucleic acid template is present, the PCR reaction selectively amplifies the target. The PCR amplification products are detected using agarose gel electrophoresis. Based on the results of the specific amplification fragment, the presence of porcine circovirus-derived components in the test sample can be determined, enabling qualitative analysis of the test results.

Kit Components:
TTSuV-2 Amplification Mix: 50 µL × 1 tube
TTSuV-2 Reaction Mix: 950 µL × 1 tube
TTSuV-2 Positive Control: 50 µL × 1 tube
TTSuV-2 Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Ready-to-use: Only DNA samples are required from the user for immediate application.
High sensitivity: Optimized primers and probes ensure reliable detection.
Includes positive control to help identify false-negative results.
High specificity: Primers are designed based on highly conserved regions of Porcine Torque Teno Virus Type 2 (TTV2) DNA to prevent cross-reactivity with other microbial DNA.
Supports both qualitative and quantitative detection; linear quantification range spans at least 5 orders of magnitude.
Sufficient for 50 reactions using a 2 μL probe-based qPCR system.
For research use only.

STEM-SLSR-0023-ZJF
STEM-SLSR-0024-ZJF
STEM-SLSR-0025-ZJF
STEM-SLSR-0026-ZJF