Porcine-Derived Component Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0028-ZJF)

This assay kit applies SYBR Green dye-based real-time PCR to amplify and detect Porcine-Derived Component nucleic acids. Specific primers are designed to target conserved regions of the target gene. Fluorescence signals emitted during amplification are recorded in real time, supporting accurate identification and measurement of Porcine-Derived Component in experimental and routine laboratory settings.

Kit Components:
Porcine-Derived Component Amplification Mix: 50 µL × 1 tube
Porcine-Derived Component Reaction Mix: 950 µL × 1 tube
Porcine-Derived Component Positive Control: 50 µL × 1 tube
Porcine-Derived Component Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Product Features
Ready-to-use format requiring only user-supplied DNA template.
High specificity: Primers are designed based on highly conserved regions of porcine-derived components and do not amplify non-porcine DNA.
Optimized primers and amplification system offer sensitivity 100 times higher than conventional PCR.
Includes non-infectious PCR positive control to help distinguish false-negative samples.
One-tube fluorescence PCR workflow minimizes the risk of environmental contamination.
For research use only; kit provides sufficient reagents for 50 PCR reactions using a 2 μL system.

STEM-SLSR-0026-ZJF
STEM-SLSR-0027-ZJF
STEM-SLSR-0029-ZJF
STEM-SLSR-0030-ZJF