Partridge-Derived Component Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0034-ZJF)

This kit utilizes SYBR Green dye-based real-time fluorescent PCR technology. Specific primers are designed to target highly conserved regions of the Partridge-Derived Component gene. In the presence of the target nucleic acid template, PCR amplification occurs, generating fluorescence signals. The fluorescence intensity is monitored in real time, enabling qualitative and quantitative analysis of Partridge-Derived Component with high sensitivity and specificity.

Kit Components:
Partridge-Derived Component Amplification Mix: 50 µL × 1 tube
Partridge-Derived Component Reaction Mix: 950 µL × 1 tube
Partridge-Derived Component Positive Control: 50 µL × 1 tube
Partridge-Derived Component Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Ready-to-use kit requiring only user-supplied DNA samples.
Primers are designed based on national standard GB/T 21105-2007, enabling highly specific detection of canine-derived components without cross-reactivity to non-canine DNA.
Fluorescent quantitative PCR offers significantly higher sensitivity than conventional PCR.
Fast detection: the entire process (per sample) can be completed within 2.0 hours.
Closed one-tube operation reduces the risk of cross-contamination.
Includes positive reference standard for accurate result interpretation.
Detection limit for canine-derived components in mixed samples is 0.01%; nucleic acid detection limit is 0. ng/µL.
For research use only; sufficient for 50 qPCR reactions with a 20 μL system volume.

STEM-SLSR-0032-ZJF
STEM-SLSR-0033-ZJF
STEM-SLSR-0035-ZJF
STEM-SLSR-0036-ZJF