The M-Blot T1000 is a rapid protein-transfer system for Western Blot experiments. It transfers proteins from polyacrylamide gels to PVDF or NC membranes. The system combines the uniformity of wet transfer with the speed and efficiency of semi-dry transfer. A semi-dry sandwich structure improves current utilization. Fresh transfer solution is continuously pumped into the filter-paper sandwich to maintain ion concentration. This approach supports efficient transfer of both small and large proteins. Typical transfer time is stated as 10-15 minutes, while the overview states completion within 20 minutes. The instrument uses approximately 100 mL of solution per run and provides touchscreen operation, monitoring, logs, cleaning programs, networking, and voice notifications.
Specification
Transfer time: 10-15 min stated in features and within 20 min stated in overview Membranes: PVDF and NC Gel type: Polyacrylamide gel Transfer structure: Semi-dry sandwich with continuous buffer replenishment Solution consumption: Approximately 100 mL per run Monitoring: Real-time voltage and current Software: Stored programs, automatic parameter recommendation, operation logs, online updates, and data collection Cleaning: Preset tubing-cleaning program User interface: Full touchscreen with voice completion reminder Reagents: Open system with optimized dedicated solutions recommended
Features
Rapid 10-20-minute transfer PVDF and NC compatibility Continuous fresh-buffer supply Stable voltage and current Small- and large-protein transfer Low 100 mL reagent use Touchscreen software control Logs, cleaning, and voice alerts