IMAC Seplife FF NTA Chelating Medium, ≥370 cm/h (CAT#: STEM-BC-3147-ZJF)

This immobilized-metal affinity chromatography medium is designed for purification of metal-binding biomolecules. It uses a high-flow agarose matrix functionalized with a chelating ligand. The source model identifies the medium as NTA, while the supplied description refers to an iminodiacetic-acid group. After loading with a suitable metal ion, the medium can interact with histidine-containing molecules. It is intended for purification of His-tagged recombinant proteins. The spherical particles provide uniform column packing and reproducible flow. High-flow operation supports efficient processing with relatively low backpressure. Broad pH stability and compatibility with selected aqueous reagents support repeated use.

Model: IMAC Seplife FF(NTA)
Appearance: Spherical gel, odorless and tasteless
Matrix: Seplife 6FF
Shape: Spherical
Model-designated ligand: NTA
Source description: Iminodiacetic-acid chelating group
Particle size: 45-165 µm
Maximum flow rate: ≥370 cm/h
Pressure resistance: 0.3 MPa
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 0.1 M sodium hydroxide, 0.1 M hydrochloric acid, and 8 M urea
Application: His-tagged recombinant protein purification

Immobilized-metal affinity medium
≥370 cm/h flow
High-flow agarose matrix
Broad pH stability
Uniform spherical particles
Compatible with 8 M urea
Suitable for His-tagged proteins
Reusable chelating platform

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