IMAC Seplife FF IDA Chelating Medium, ≥370 cm/h (CAT#: STEM-BC-3148-ZJF)

This immobilized-metal affinity chromatography medium is designed for purification of His-tagged recombinant proteins. It uses a high-flow agarose matrix functionalized with iminodiacetic-acid groups. The IDA ligand can chelate suitable metal ions for affinity capture. Histidine residues on target proteins interact with the immobilized metal during loading. The medium can serve as a reusable platform for compatible metal-affinity processes. Spherical particles support uniform packing and consistent chromatographic performance. A maximum flow rate of at least 370 cm/h enables efficient sample processing. Broad pH stability and compatibility with selected aqueous solutions support repeated purification cycles.

Model: IMAC Seplife FF(IDA)
Appearance: Spherical gel, odorless and tasteless
Matrix: Seplife 6FF
Ligand: Iminodiacetic acid
Shape: Spherical
Particle size: 45-165 µm
Maximum flow rate: ≥370 cm/h
Pressure resistance: 0.3 MPa
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 0.1 M sodium hydroxide, 0.1 M hydrochloric acid, and 8 M urea
Application: His-tagged recombinant protein purification

IDA chelating functionality
Selective metal-affinity capture
≥370 cm/h flow
High-flow agarose matrix
Broad CIP pH range
Uniform particle distribution
Urea-compatible operation
Suitable for recombinant proteins

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