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IL-3 Detection (CAT#: STEM-MB-0272-WXH)

Introduction

IL-3 is a hematopoietic growth factor and is mainly produced by activated T cells. It can promote the proliferation of early hematopoietic progenitor cells. Besides, IL-3 can also promote the differentiation of hematopoietic progenitor cells together with erythropoietin granulocyte colony-stimulating factor(G-CFS), and macrophage colony-stimulating factor(M-CFS). It is important for the body's hematopoietic and immune regulatory functions. In addition, IL-3 can also participate in the development of the central nervous system, promote the proliferation and survival of neural precursor cells, then affect brain capacity. In the clinic, the combination of IL-3 with GM-CFS, G-CSF, M-CSF, or EPO after chemotherapy can appropriately stimulate the production of blood cells and platelets, which can rebuild bone marrow function when bone marrow suppression caused by chemotherapy and radiotherapy. Besides, the combination can also improve aplastic anemia. In diseases, IL-3 can also regulate eosinophils and other inflammatory cells, and it is the key factor to start and sustain asthma airway inflammation.




Principle

The IL-3 receptor is a heterodimer composed of two chains of α and β, with a molecular weight of 140 kD. The α chain is unique to IL-3, and also determines the specificity of IL-3 action. The β chain is shared with GM-CSF and IL-5. Among them, only a single α chain can form a high affinity receptor. The signal transduction of IL-3 in the regulation of hematopoietic stem cell proliferation and differentiation is mainly achieved through the JAK2 and Src kinases. When IL-3 functions, IL-3 first forms heterodimers or oligomers. After the oligomers polymerize, it stimulates intracellular signal transduction. The IL-3R cytoplasm interacts with the Scr family tyrosine kinases such as Lyn and Fyn, thereby activating the ras-MAPK pathway. MAPK can activate STAT and act on promoters of related genes, thereby regulating related gene expression.

Applications

IL-3 can promote the proliferation and differentiation of early hematopoietic progenitor cells.
IL-3 can participate in the development of the central nervous system, promote the proliferation and survival of neural precursor cells, then affect brain capacity.
In the clinic, IL-3 can rebuild bone marrow function when bone marrow suppression caused by chemotherapy and radiotherapy.

Procedure

1. Process samples.
2. IL-3 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Notes

Sample Types: Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.

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