Glycanassure™ Hyperformance APTS Kit, Ex: 455 nm, Em: 500 nm, Thermo Fisher Scientific (CAT#: STEM-AS-0274-LSY)

Highlights

Simple N-glycan sample preparation workflow for LC and CE analysis platforms
Excellent data quality and unparalleled robustness
Proprietary denaturant, which can completely deglycosylate glycoprotein

Cat Number: STEM-AS-0274-LSY

Application: For glycosylation analysis

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Description

Glycanassure™ Hyperformance APTS Kit, Ex: 455 nm, Em: 500 nm, model A33952 is manufactured by Thermo Fisher Scientific. The GlycanAssure HyPerformance APTS kit is a rapid N-glycan release, labeling and removal kit, which can provide N-glycan sample preparation workflow for high-throughput glycan characterization applications in biopharmaceuticals.
GlycanAssure HyPerformance APTS uses a proprietary denaturant to achieve complete glycosylation, which can well preserve sialylation (an important quality attribute of glycoproteins), and allows analysis on the CE instrument, which is highly sialylated at the beginning of the electrophoresis instrument Glycans will migrate to achieve accurate quantification.
The Applied BioSystems 3500xL genetic analyzer for protein quality analysis for high-throughput applications or the Thermo Scientific Vanquish Flex UHPLC system for low-throughput applications can be used to analyze the released labeled glycans for use in Thermo Fisher Scientific end-to-end Teloglycan analysis solution. The GlycanAssure HyPerformance APTS kit contains the following components:
GlycanAssure core reagent
GlycanAssure magnetic beads
GlycanAssure APTS labeling reagent
GlycanAssure Suite User Guide

Specification

Excitation/Emission: EX: 455 nm, Em: 500 nm
Shipping Condition: Dry Ice
Label Type: Classic Dyes
Label or Dye: Pyrene
Reactive Moiety: Aldehyde, Ketone
Number: A33952
Unit specifications: 96 reactions
Contents and storage: GlycanAssure Buffer Kit, store at room temperature
GlycanAssure Bead Kit, store at 2–8°C
Glycan Assure Core Kit, store at -5 to -30°C

Features

Simple N-glycan sample preparation workflow for LC and CE analysis platforms
Excellent data quality and unparalleled robustness
Proprietary denaturant, which can completely deglycosylate glycoprotein
The retention of sialylation provides accurate quantification of sialylated glycans
High-throughput end-to-end N-glycan analysis greatly shortens the time to obtain results

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