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Characterizing of nanocarriers by Circular dichroism (CD) (CAT#: STEM-MB-0615-WXH)

Introduction

CD studies are usual for characterizing of nanocarriers as potential gene/drug delivery devices in nanomedicine and their interactions with biomolecules and drugs have attracted considerable attention in recent studies. Not only CD often allows one to obtain structural information on the assembled structure of nanocarriers, but also, the CD spectra can predict the efficiency of such nanomaterials in packaging a gene-construct for delivery to a host cell. In addition, conformation analysis of a DNA, before (or after) packaging in a nanocarrier can be monitored by CD.




Principle

Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.

Applications

Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.

Procedure

1. Sample preparation
2. Measurement by CD instrument
3. Data analysis

Materials

Circular dichroism (CD) spectrophotometer