Candidatus Liberibacter asiaticus (Oleander Strain) Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1334-ZJF)

This kit utilizes probe-based real-time fluorescent PCR technology. Specific primers are designed to target highly conserved regions of the Candidatus Liberibacter asiaticus (Oleander Strain) gene. The system enables in vitro amplification and detection of Candidatus Liberibacter asiaticus (Oleander Strain) nucleic acids. When the target nucleic acid is present, the PCR reaction proceeds and generates fluorescence signals. These signals are monitored in real time by the instrument to provide both qualitative and quantitative results.

Kit Components:
Candidatus Liberibacter asiaticus (Oleander Strain) Amplification Mix: 50 µL × 1 tube
Candidatus Liberibacter asiaticus (Oleander Strain) Reaction Mix: 950 µL × 1 tube
Candidatus Liberibacter asiaticus (Oleander Strain) Positive Control: 50 µL × 1 tube
Candidatus Liberibacter asiaticus (Oleander Strain) Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

No need for additional preparation; add DNA and start.
Specific detection enabled by primers/probes based on conserved sequences.
Assay components are fine-tuned for high sensitivity.
Positive control included to verify test reliability.
Closed-system qPCR protocol helps prevent contamination.
Time-efficient workflow for quick results.
Intended solely for scientific investigations.

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