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Barnes Maze (CAT#: STEM-AE-0017-WXH)

Introduction

The Barnes maze is a model invented by American scholar Carol ABarnes in 1979 to test the spatial memory of animals. Similar to the water maze and the radial arm maze, the Barnes maze was established using the characteristics of rodents that avoid light and love to explore. Animals were augmented by fleeing from above a lighted, open platform to a dark, cramped enclosure below the platform. This bin is called the target bin. After training, the animals learn and memorize the location of the target box. The model is less stressful for the animals, neither requiring fasting like the radial arm maze nor being as stressful as the water maze. Therefore, it is more commonly used in memory research. Especially useful for stress-related memory studies and behavioral phenotype studies in knockout mice.




Applications

• Measuring Spatial Reference Memory and Working Memory in Animals.
• Stress-related memory studies and behavioral phenotypes in knockout mice.

Procedure

1. Adapt.
2. Put the animal in a plastic bucket in the center of the maze and restrict its activities for 5s.
3. Remove the barrel and start the timer. If all the limbs of the animal entered the target box, it was counted as escape, and the animal remained in the box for 30 seconds. Observe each animal once for 4 minutes each. During this time, if the animal still cannot find the target box, move the animal out of the maze and into the target box for 30 s. Use this gap to clear the maze. Animals were trained twice a day for 5-6 days.
4. The experiment recorded the following parameters: latency to explore any hole, latency to reach the target box, number of mistakes made by each animal.

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