Acquired Helplessness Experiment (CAT#: STEM-AE-0013-WXH)

Research on the Pathogenesis of Depression.
Research and development of new drugs and healthcare products.

1. Model building period
a: On the day of the experiment, the animals should be put into the test box and allowed to adapt for 5 minutes.
b: Animals should receive cyclic electric shocks for 30-60 operating cycles in the electric shock box. Each operation cycle should include a double-chamber plantar electric shock period and an intermission period in which there is no signal and cannot be escaped. The electric shock time should be 3-10s, and the intermission period should be 3-10s.
2. Conditioned avoidance test period
a: The conditioned avoidance test should be performed on the second day after the model is established.
b: The animal is put into the test box, and after 5 minutes of adaptation, the experiment is carried out for 15 to 30 operating cycles.
c: The total time of each running cycle should be 30s, including 3-10s conditioned stimulus (light/sound), 3-10s conditioned stimulus + unconditioned stimulus (electric shock condition is the same as before) and 5-25s intermission period (no give any stimulus).
d: Repeat the above steps for 2~3 consecutive days.