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Analysis of CAPN10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1547-LGZ)

Name: Analysis of CAPN10 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1547-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: calpain 10
Also known as: CANP10; NIDDM1
Calpains are a ubiquitous and well conserved family of calcium-dependent cysteine proteases. The calpain protein is a heterodimer composed of an invariant small subunit and a variable large subunit. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; -binding domain. This gene encodes a large subunit. It is an atypical calpain because it lacks a calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is organizationally similar to calpain5 and calpain6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and is located in the NIDDM1 region. Multiple alternative transcript variants of this gene have been described.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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