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Determination of cobalt in water samples by Atomic absorption spectroscopy (AAS) (CAT#: STEM-ST-0166-WXH)

Introduction

Cobalt is known to be essential at trace levels to man, animals and plants for metabolic processes. The determination of trace amounts of cobalt in natural waters is of great interest because cobalt is important for living species as complexed Vitamin B12. The deficiency of cobalt in ruminants usually results in different types of anemia. Toxicological effects of large amounts of cobalt include vasodilation, flushing and cardiomyopathy in humans and animals. Investigations have been extended to the biochemistry of cobalt in animals, microorganisms and enzymes.




Principle

Atomic absorption spectrometry (AAS) detects elements in either liquid or solid samples through the application of characteristic wavelengths of electromagnetic radiation from a light source.
Atomic absorption spectroscopy (AAS) is based upon the principle that free atoms in the ground state can absorb light of a certain wavelength. Absorption for each element is specific, no other elements absorb this wavelength.

Applications

Atomic absorption spectrometry (AAS) is an easy, high-throughput, and inexpensive technology used primarily to analyze elements in solution. As such, AAS is used in food and beverage, water, clinical research, and pharmaceutical analysis.

Procedure

1. Creating a steady state of freely dissociated ground state atoms using a heat source (flame)
2. Passing light of a specific wavelength through the flame. The wavelength corresponds to the amount of energy required to excite an electron from (typically) the ground to first excited state for a specific element.
3. Measuring the amount of the light absorbed by the atoms as they move to the excited state (the atomic absorption).
4. Using the measured absorbance to calculate the concentration of the element in a solution, based on a calibration graph.

Materials

• Spectrometer
• Radiation sources
• Atomizers
• Atomic absorption
• Spectrophotometer