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Study of Spherical Bilayer Vesicles of Fullerene-Based Surfactants in Water by Laser Light Scattering (CAT#: STEM-ST-0161-YJL)

Introduction

The extremely hydrophobic C60 fullerene can be made soluble in water by connecting it with functional chargeable groups such as carboxylic acids or amines. Hydrophilic behavior can also be introduced by an elegant and less obvious approach, one in which polarizable phenyl groups are added to C60 to stabilize its anion. Because these compounds are composed solely of carbon and hydrogen atoms, they represent a rare example of a hydrophobic hydrocarbon ball linked to a hydrophilic ion, causing the pentasubstituted fullerene to achieve a surfactant functionality. The low solubility of fullerenes in aqueous solution limits their applications in biology. By appropriate substitution, the fullerenes can be transformed into stabilized anions that are water soluble and can form large aggregated structures.




Principle

Laser scattering method is a commonly used material detection method, which uses a laser beam to irradiate the sample, and analyzes the physical and chemical properties of the sample by detecting the intensity and direction of the scattered light from the sample. This method has the advantages of non-contact, high sensitivity, and high resolution, and is widely used in materials science, biomedicine, and environmental monitoring.
The detection principle of the laser scattering method is based on the scattering phenomenon of light. When the laser beam hits the surface of the sample, part of the light will be scattered in all directions, forming scattered light. The intensity and direction of these scattered light are related to the physical and chemical properties of the sample, so the properties of the sample can be analyzed by detecting the characteristics of the scattered light.

Applications

Laser light scattering (LLS) is used to determine size of various particles including proteins, polymers, micelles and nanoparticles.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Laser Light Scattering Instrument