Determination of Absolute Molecular Mass of Biomolecules by Laser Scattering (CAT#: STEM-ST-0163-YJL)

Introduction

When the macromolecules in the solution are irradiated by a beam of laser light, the charges in the molecules vibrate under the influence of the electric field of the light, resulting in scattered light. The scattered light intensity is proportional to the quadratic (E2) of the electric field intensity, and incoherent light is generated between free molecules, and the scattered light intensity is proportional to (E12+E22). While the molecular complexes bound together produce coherent light, the intensity of scattered light is proportional to (E1+E2)2. Based on this principle, free A/B molecules and bound AB complexes will produce different scattering signals. During laboratory detection, the intensity of scattered light at a fixed angle is proportional to the square of the molecular weight, concentration and specific refractive index increment (dn/dc) of the substance.

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Principle Applications Procedure Materials Notes Related Products

Principle

Laser scattering method is a commonly used material detection method, which uses a laser beam to irradiate the sample, and analyzes the physical and chemical properties of the sample by detecting the intensity and direction of the scattered light from the sample. This method has the advantages of non-contact, high sensitivity, and high resolution, and is widely used in materials science, biomedicine, and environmental monitoring.
The detection principle of the laser scattering method is based on the scattering phenomenon of light. When the laser beam hits the surface of the sample, part of the light will be scattered in all directions, forming scattered light. The intensity and direction of these scattered light are related to the physical and chemical properties of the sample, so the properties of the sample can be analyzed by detecting the characteristics of the scattered light.

Applications

Laser light scattering (LLS) is used to determine size of various particles including proteins, polymers, micelles and nanoparticles.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Laser Light Scattering Instrument

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