Study of Effect of Charge Ratio on the Properties of Folate-chitosan-DNA Nanoparticles by Laser Scattering (CAT#: STEM-ST-0162-YJL)

Introduction

Chitosan is a non-toxic biodegradable polycationic polymer with low immunogenicity. The chitosan molecule is a polysaccharide copolymer of N-acetyl-d-glucosamine and d-glucosamine. Its cationic polyelectrolyte nature provides a strong electrostatic interaction with negatively charged DNA, and protects it from nuclease degradation. This makes chitosan a good candidate for non-viral gene delivery. Folic acid (FA) is appealing as a ligand for targeting cell membrane and allowing nanoparticle endocytosis via the folate receptor (FR) for higher transfection yields. Importantly, the high affinity of folate to bind its receptor (1 nm) and folate's small size allows its use for specific cell targeting.

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Principle Applications Procedure Materials Notes Related Products

Principle

Laser scattering method is a commonly used material detection method, which uses a laser beam to irradiate the sample, and analyzes the physical and chemical properties of the sample by detecting the intensity and direction of the scattered light from the sample. This method has the advantages of non-contact, high sensitivity, and high resolution, and is widely used in materials science, biomedicine, and environmental monitoring.
The detection principle of the laser scattering method is based on the scattering phenomenon of light. When the laser beam hits the surface of the sample, part of the light will be scattered in all directions, forming scattered light. The intensity and direction of these scattered light are related to the physical and chemical properties of the sample, so the properties of the sample can be analyzed by detecting the characteristics of the scattered light.

Applications

Laser light scattering (LLS) is used to determine size of various particles including proteins, polymers, micelles and nanoparticles.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Laser Light Scattering Instrument

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