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Study of characterization of the Spatial Configuration of Microbial Products in Aqueous Solution by Laser Scattering (CAT#: STEM-ST-0164-YJL)

Introduction

Microbial products, including extracellular polymeric substances (EPS) and soluble microbial products (SMP, or soluble EPS), are high-molecular weight compounds excreted by the microorganisms grown in suspended cultures or microbial aggregates, such as biofilm, flocs, and granular. EPS are the polymeric substances located at or outside the cell surface, while SMP are the soluble cellular polymeric components dissolved in aqueous solution, irrespective of their origins. The composition of microbial products can be controlled by different metabolisms, chemical, and physical processes: EPS may come from active secretion of living cells, shedding of cell surface material, death and lysis of cells, and adsorption from the environment, while SMP are released during microbial metabolism, cell lysis, and EPS hydrolysis. The structural characteristics can help better understand the relationship between the microscopic structure of microbial products and their macroscopic properties (e.g., adsorption, flocculation, and adhesion).




Principle

Laser scattering method is a commonly used material detection method, which uses a laser beam to irradiate the sample, and analyzes the physical and chemical properties of the sample by detecting the intensity and direction of the scattered light from the sample. This method has the advantages of non-contact, high sensitivity, and high resolution, and is widely used in materials science, biomedicine, and environmental monitoring.
The detection principle of the laser scattering method is based on the scattering phenomenon of light. When the laser beam hits the surface of the sample, part of the light will be scattered in all directions, forming scattered light. The intensity and direction of these scattered light are related to the physical and chemical properties of the sample, so the properties of the sample can be analyzed by detecting the characteristics of the scattered light.

Applications

Laser light scattering (LLS) is used to determine size of various particles including proteins, polymers, micelles and nanoparticles.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Laser Light Scattering Instrument