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Structural analysis of minerals and aligned collagen fibrils in tilapia fish scales by dark-field and energy-filtered transmission electron microscopy and electron tomography technology (CAT#: STEM-MIT-0031-LJX)

Introduction

Tilapia scales are the protective scales on its surface, usually in the shape of a fan. The inside of the fish is a tight fiberboard layer structure, and a layer of biofilm structure, with hydroxyapatite as the main component of inorganic substances mainly distributed in the surface of the fish scale.




Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Tilapia fish scales

Notes

Pay attention to air humidity
Voltage needs to be stabilized