Investigation of DNA structures by scanning tunneling microscope (CAT#: STEM-MIT-0322-LJX)

Name: Investigation of DNA structures by scanning tunneling microscope
SKU: STEM-MIT-0322-LJX
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Introduction

DNA or deoxyribonucleic acid carries the genetic information necessary for the synthesis of RNA and proteins and is a biomacromolecule essential for the development and proper functioning of living organisms.
The primary structure of DNA refers to the connection of four nucleotides and their sequence, which represents the chemical composition of the DNA molecule. The secondary structure of DNA is the double helix structure formed when two polynucleotide chains are coiled in parallel and opposite directions.

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Principle Applications Procedure Materials Notes

Principle

The basic principle of scanning tunneling microscope (STM) is to use the tunneling effect in quantum theory. An extremely fine probe of atomic linearity and the surface of the substance under study are used as two electrodes. When the sample is very close to the tip of the needle (usually less than 1nm), electrons flow through the barrier between the two electrodes to the other electrode under the action of an applied electric field. This phenomenon is known as tunneling effect.

Applications

Studing the structure of the sample surface by high resolution imaging
Manipulating individual atoms to form nanostructures

Procedure

1. Sampling
2. Preparation of slices (Select according to the specific experimental situation)
3. Staining (Select according to the specific experimental situation)
4. Observation