Fiber FISH (CAT#: STEM-MB-1177-WXH)

Introduction

Fiber-FISH is defined as the application of fluorescence in situ hybridization (FISH) techniques on extended DNA molecules. In most cases, the target DNA is prepared by deproteinizing chromosomal chromatin by extraction with detergents or high salt concentration. This treatment removes histones from chromatin and leads to a complete unwinding of the DNA to the level of a linear double helix of approx 340 nm/1000 bp.<br />Fiber FISH provides a higher resolution of analysis than conventional FISH and yields more precise information as to the localization of a specific DNA probe on the chromosome.<br />The word "fiber" here refers not to dietary fiber but to a chromosome fiber.