Assessing the Photo-degradation of Biopharmaceutical Compounds by FTIR (CAT#: STEM-B-0397-CJ)

In FTIR analyses, Infrared light from the light source passes through a Michelson interferometer along the optical path.
The interferometer comprises a beam splitter, moving mirror, and fixed mirror. The light beam split into two by the beam splitter is reflected from the moving mirror and fixed mirror, before being recombined by the beam splitter. As the moving mirror makes reciprocating movements, the optical path difference to the fixed mirror changes, such that the phase difference changes with time. The light beams are recombined in the Michelson interferometer to produce interference light. The intensity of the interference light is recorded in an interferogram, with the optical path difference recorded along the horizontal axis.

Biopharmaceutica

1. Sample preparation.
2. (sub)visible particles present in a sample are first isolated on a filter, preferably a metal-coated membrane or a gold filter.
3. bright field images are taken from the particles and IR spectra are obtained for each individual particle.
4. Data analysis.

• Sample: Peptides, Proteins, Vaccines, Virus-like particles
• Equipment: FTIR spectroscopy