Assessing the Hydrolysis of Biopharmaceutical Compounds by Reversed-Phase Chromatography (RP-HPLC / RP-UPLC) (CAT#: STEM-B-0411-CJ)

Reversed-phase chromatography on high-performance liquid chromatography (HPLC) instruments coupled with UV-, fluorescence-, refractive index- and/or multi-angle laser light scattering (MALLS) detection. Further, ultra-high-performance chromatography (UPLC) instruments with UV detection are available, reducing analysis times, requiring lower sample and mobile phase volumes and delivering better resolution.

Biopharmaceutica

1. Sample preparation.
2. Binding an organic molecule to a stationary phase,
3. The hydrophobic binding of the solute molecule from the mobile phase to the immobilized hydrophobic ligands attached to the stationary phase, i.e., the sorbent.
4. Eluting the organic molecule using a gradient of a less polar organic solvent: Elution can proceed either by isocratic conditions where the concentration of organic solvent is constant, or by gradient elution whereby the amount of organic solvent is increased over a period of time.

• Sample: Peptides, Proteins, Vaccines, Virus-like particles
• Equipment: HPLC & UHPLC Columns