Assessing the Photo-degradation of Biopharmaceutical Compounds by HPLC-DAD (CAT#: STEM-B-0399-CJ)

cIEF is based on the principle of capillary gel electrophoresis (cGE). In general, electrophoresis is a separation technique based on the migration of charged molecules in response to an electric field, toward the electrode of opposite charge. It is performed mainly in polyacrylamide gels. To separate molecules based on pI, a pH gradient inside the gel is established by ampholyte mixtures. The molecule of interest migrates along the electrical field until it reaches the pH corresponding to its pI, where it has a net charge of zero and stops migrating. The UV absorption over the whole capillary is measured throughout the separation, allowing real-time observation as well as a final quantification.

Biopharmaceutica

1. Sample preparation.
2. The sample is injected at the sample injection. The high pressure pump drives the solvent to carry the sample to go through the column with absorbent. Different compounds will receive different resistance when they go through the column.
3. Download and analyse data.

• Sample: Peptides, Proteins, Vaccines, Virus-like particles
• Equipment: HPLC system equipped with DAD