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Study of bacteriophage structure by Circular dichroism (CD) (CAT#: STEM-MB-0605-WXH)

Introduction

Bacteriophages, also known as phages, are viruses that infect and replicate only in bacterial cells. They are ubiquitous in the environment and are recognized as the most abundant biological agent on earth. The bacteriophage consists of a polyhedral head, a short collar and a helical tail. Head-The head consists of 2000 capsomeres with double-stranded DNA enclosed within. Tail-The tail consists of an inner hollow tube which is surrounded by a contractile sheath with 24 annular rings.




Principle

Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.

Applications

Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.

Procedure

1. Sample preparation
2. Measurement by CD instrument
3. Data analysis

Materials

Circular dichroism (CD) spectrophotometer