Simultaneous Cu-, Fe-, and Zn-specific detection of metalloproteins by ICP-AES (CAT#: STEM-ST-0176-WXH)

Name: Simultaneous Cu-, Fe-, and Zn-specific detection of metalloproteins by ICP-AES
SKU: STEM-ST-0176-WXH
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Introduction

All organisms must regularly ingest sufficient quantities of essential trace elements, such as Cu, Fe, and Zn, to maintain the continuous in vivo assembly of biologically active metalloproteins, which are inherently associated with health.
Analytical methods which are capable of determining the plasma or serum metalloproteome have inherent diagnostic value for human diseases associated with increased or decreased concentrations of specific plasma metalloproteins.

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Principle Applications Procedure Materials

Principle

Atomic emission spectroscopy (AES) is a method of chemical analysis that uses the intensity of light emitted from a flame, plasma, arc, or spark at a particular wavelength to determine the quantity of an element in a sample. The wavelength of the atomic spectral line in the emission spectrum gives the identity of the element while the intensity of the emitted light is proportional to the number of atoms of the element. The sample may be excited by various methods.
Inductively coupled plasma atomic emission spectroscopy (ICP-AES) uses an inductively coupled plasma to produce excited atoms and ions that emit electromagnetic radiation at wavelengths characteristic of a particular element.

Applications

Common applications of atomic emission spectroscopy are in the analysis of trace elements in soils, water, metals, biological specimens, clinical specimens, food, physical evidence such as glass, and other solids.

Procedure

1. Prepare sample for analysis
2. Convert solution into aerosol
3. Introduce aerosol into excitation source
4. Atomization and excitation
5. Detection of emission lines