Protein Purification by HPLC (CAT#: STEM-MB-1296-LGZ)

Introduction

Chromatography is a general analytical technique used to separate a mixture into its individual components. High performance liquid chromatography, commonly known as HPLC, has a variety of applications in the chemical biology research laboratory.<br />In chemical biology, individual analytes, such as peptides, are often chromatographically purified for use as a functional tool. High performance liquid chromatography (HPLC) is a method used to analyze and separate liquid samples. In chemical biology laboratories, HPLC is considered indispensable for the purification of peptides (synthesized manually or automated with a synthesizer) and other small to medium-sized organic molecules. It also allows you to use a very much smaller particle size for the column packing material which gives a much greater surface area for interactions between the stationary phase and the molecules flowing past it. This allows a much better separation of the components of the mixture.<br />There are many types of HPLC columns developed for specific applications such as normal-phase HPLC (NP-HPLC) and reversed phase HPLC (RP-HPLC). The right choice of column is critical for obtaining good HPLC results. Column choice is governed by characteristics of components in the mixture we wish to separate.