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Primer Synthesis (Oligonucleotide Synthesis) (CAT#: STEM-MB-0004-WXH)

Introduction

Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence.




Applications

• Used as antisense oligonucleotides
• Used as small interfering RNA
• Primers for DNA sequencing and amplification,
• Probes for detecting complementary DNA or RNA
• Tools for the introduction of mutations
• Synthesis of artificial genes

Procedure

1. Remove the protective group of the 5-hydroxyl group
2. Raw materials for DNA synthesis
3. Capped reaction
4. Repeat the above steps
5. Purification

Notes

Customer provides primer synthesis information sheet.