Genomic DNA Extraction (CAT#: STEM-MB-0003-WXH)

Introduction

Genomic DNA extraction is the process of releasing chromosomal DNA from the cellular matrix in which it is contained. Genomic DNA extraction requires a robust disruption method to open the nuclei and cell walls; it usually involves adding a compatible detergent as well as mechanical shearing.<br />Genomic DNA extraction is the process of releasing chromosomal DNA from the cellular matrix that contains it. Genomic DNA extraction requires a robust disruption method to open the nucleus and cell wall; it usually involves the addition of compatible detergents as well as mechanical shearing.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Preparation of vaccines
• DNA recombination experiment
• DNA library construction
• Southern hybridization
• Studying the Genetic Factors of Disease
• Genome sequencing
• Detect bacteria and viruses in the environment
• Determine the parent-child relationship

Procedure

1.Disruption of the cell structure to create a lysate
2. Protection of DNA from degradation during processing
3.Separation of the soluble DNA from cell debris and other insoluble material
4.Elution of purified DNA.

Notes

Customers provide fresh or properly preserved samples.

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