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Lentivirus Vector Construction/Lentivirus Packaging (CAT#: STEM-MB-0006-WXH)

Introduction

Recombinant lentivirus is the most commonly used viral vector for efficient gene delivery into mammalian cells. Unlike plasmid DNA vector which only allows transient and episomal expression of the foreign gene in the host cell, lentiviral vector can achieve permanent expression of the foreign gene through integration into the host cell genome. Lentivirus is also efficient for in vivo gene delivery.




Principle

Lentiviruses (a genus of retrovirus) express reverse transcriptase, which converts the viral RNA to double-stranded DNA, and integrase, which inserts this viral DNA into the host DNA.

Applications

• In vivo gene delivery
• Gene therapy

Procedure

1. Insert fragment
2. Co-transfection
3. Supernatant collection
4. Concentration and purification