Pasmid Extraction (CAT#: STEM-MB-0001-WXH)

Introduction

Plasmids are a class of autonomously replicating genetic components that exist in the cytoplasm and are independent of chromosomes. Plasmid extraction refers to the removal of RNA, the separation of plasmids and bacterial genomic DNA, and the removal of proteins and other impurities to obtain relatively pure plasmids. There are three commonly used plasmid extraction methods: alkaline lysis, boiling and detergent lysis.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Used as a vector in recombinant DNA technology
• Gene therapy
• Production of Drugs and Proteins
• Gene transfer
• Gene knockout

Procedure

1.Growth of the bacterial culture
2.Harvesting and lysis of the bacteria
3.Purification of plasmid DNA

Notes

Customers need to provide the target plasmid or the corresponding bacterial liquid.

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