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Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each target. This technique requires two or more probes that can be distinguished from each other and detected simultaneously.<br />Advantages of using multiplex PCR include:<br />More information with less sample<br />Higher throughput<br />Cost effective — fewer dNTPs, enzymes, and other consumables<br />Time saving<br />Less input material required<br />More data from limited starting materials<br />Increased accuracy of data normalization<br />Fewer pipetting errors