Monitoring autophagy of mammalian cells by transmission electron microscopy technology (CAT#: STEM-MIT-0026-LJX)

Introduction

Autophagy is a kind of "self-feeding" phenomenon, through the degradation of damaged or excess proteins, organelles and other cytoplasmic components in the cell, so as to achieve the metabolic needs of the cell itself and the renewal of organelles. Under normal circumstances, autophagy helps maintain cell homeostasis and prevent the accumulation of damaged proteins or organelles in the cell. Through the formation and extension of phagocytic vesicles, autophagosomes are generated and then fused with lysosomes to form autophagolysosomes, which degrade cell polymers and damaged organelles and provide nutrients and energy for cell renewal.

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Principle Applications Procedure Materials Notes Related Products

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Mammalian cells

Notes

Pay attention to air humidity
Voltage needs to be stabilized

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