Analysis of twin-arginine translocase (Tat) by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0803-WXH)

Introduction

The twin-arginine translocase (Tat) complex is a unique system that translocates folded proteins across the cytoplasmic membrane. The twin-arginine translocation pathway (Tat pathway) is a protein export, or secretion pathway found in plants, bacteria, and archaea. In contrast to the Sec pathway which transports proteins in an unfolded manner, the Tat pathway serves to actively translocate folded proteins across a lipid membrane bilayer. In plants, the Tat translocase is located in the thylakoid membrane of the chloroplast, where it acts to export proteins into the thylakoid lumen. In bacteria, the Tat translocase is found in the cytoplasmic membrane and serves to export proteins to the cell envelope, or to the extracellular space. The existence of a Tat translocase in plant mitochondria is also proposed.