Analysis of Trp53 Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0027-LGZ)

Introduction

Official Full Name: transformation related protein 53<br />Also known as: bbl; bfy; bhy; p44; p53; Tp53<br />This gene encodes the oncoprotein p53, which responds to various cellular stresses, regulates target genes, and induces cell cycle arrest, apoptosis, senescence, DNA repair or metabolic changes. The expression level of P53 protein is low in normal cells and high in a variety of transformed cell lines, which is considered to be related to transformation and malignant tumors. p53 is a DNA-binding protein containing transcriptional activation, DNA-binding and oligomerization domains. It is speculated that it acts as a tumor suppressor by binding to the p53 binding site and activating the expression of downstream genes that inhibit growth and/or invasion. Mice lacking the gene developed normally but were prone to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been de terminated .