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Target Sequencing (CAT#: STEM-MB-0073-WXH)
Introduction
Target sequencing is a technical means to use probe hybridization to enrich the DNA sequence of the exon region, and through high-throughput sequencing to discover mutations related to protein function variation. Compared with whole genome and exome sequencing, target sequencing is more economical and efficient. Target sequencing is mainly used for the study of large sample size and small regional variation, which is more conducive to the detection of low-frequency mutations, and is widely used in clinical inspection and drug use.
Applications
• Focusing on specific regions of interest
• Targeted analysis of microbial genes
• Detecting rare variants, sequence-focused content from multiple samples in parallel using multiplexing
• Achieving comprehensive coverage, yet generating a smaller, more reasonable amount of data
• Identifying causative variants like SNPs and InDels across multiple genomic regions
• Fast turnaround time for more samples
• Targeted analysis of microbial genes
• Detecting rare variants, sequence-focused content from multiple samples in parallel using multiplexing
• Achieving comprehensive coverage, yet generating a smaller, more reasonable amount of data
• Identifying causative variants like SNPs and InDels across multiple genomic regions
• Fast turnaround time for more samples
Procedure
1.DNA Extraction
2.Library construction
3.In-solution hybridization capture
4.Sequencing
5.Data analysis
2.Library construction
3.In-solution hybridization capture
4.Sequencing
5.Data analysis
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Wide range of applications
For various laboratory needs
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