Whole Genome DNA Methylation Sequencing (WGBS) (CAT#: STEM-MB-0042-WXH)

Introduction

Whole genome bisulfite sequencing（WGBS） is a next-generation sequencing technology used to determine the DNA methylation status of single cytosines by treating the DNA with sodium bisulfite before high-throughput DNA sequencing. The DNA methylation is an epigenetic mechanism to regulate gene expression and involves adding a methyl group to a cytosine base. Abnormal methylation patterns have been associated with several conditions and diseases, such as cancer. Advantages of whole genome methylation sequencing (WGBS): 1. High measurement accuracy 2. Wide detection range 3. High reliability 4. High cost performance

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Principle Applications Procedure Materials Notes Related Products

Applications

• Research on basic mechanisms of cell differentiation and tissue development
• Animal and plant breeding
• Disease treatment
• Regulation of gene expression
• Cancer research
• Epigenetic Heterogeneity Studies
• Chromatin remodeling

Procedure

1. Quality inspection of genomic DNA preparation
2. Library construction and sequencing
3. Data filtering quality control
4. Reference genome comparison
5. Data Analysis

Notes

Customer provides DNA sample.

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