Analysis of Reactive Oxygen Species (ROS) by Fluorescence Microplate Reader (CAT#: STEM-MB-1517-LGZ)

DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluoresent probe that can freely traverse cell membranes without fluorescence itself, and can be hydrolyzed into DCFH (dichlorofuorescin diacetate) by intracellular esterase. In the presence of reactive oxygen species, DCFH is oxidized into strong green fluorescein substance DCF (dichlorofluorescein), whose fluorescence has a maximum peak near the excitation wavelength of 502nm and emission wavelength of 530nm, and the intensity is proportional to the level of intracellular reactive oxygen species.

It is suitable for detecting reactive oxygen species in tissue cells and cultured cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

• Sample Type: tissue cells and cultured cells