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Analysis of Cell Copper (Cu) by Enzyme-labeled Instrument (CAT#: STEM-MB-1474-LGZ)
Introduction
Sensitivity: 0.18 μmol/L
Detection range: 0.18-5 μmol/L
Precision: inter-lot difference of 3.1%, intra-lot difference of 3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 575-585 nm
Principle
Under acidic conditions, copper ions dissociate from ceruloplasmin and react with the complexing agent to produce purple complexes with absorbance values proportional to concentration within a certain range.
Applications
It is used to detect the content of copper ion in cells.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: cell samples
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