Analysis of Aspartate Aminotransferase (AST/GOT) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1364-LGZ)

Aspartate aminotransferase (AST) catalyzes the amino conversion reaction between aspartic acid and α-ketoglutarate to generate glutamic acid and oxaloacetate, which can be decarboxylated into pyruvate by itself during the reaction. Pyruvate reacts with phenylhydrazine to form phenylhydrazone, which is reddish-brown under alkaline conditions.

It is suitable for detecting AST activity of samples such as serum (plasma), animal tissue, and cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum (plasma), animal tissue, and cells