Analysis of Cholinesterase (CHE) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1454-LGZ)

Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine, which is not hydrolyzed by cholinesterase, reacts with hydroxylamine to generate acetylhydroxylamine, which reacts to form brown red hydroxamic acid iron complex in acidic solution. The color depth is proportional to the amount of residual acetylcholine, and the comparable color quantity, thus the activity of cholinesterase can be calculated.

It is used to detect cholinesterase activity in serum (plasma) and animal tissues.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum (plasma) and animal tissues