Analysis of Sucrase Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1446-LGZ)

Sucrase acts on its substrate (sucrose) to produce glucose, glucose under the action of its grape oxidase to produce hydrogen peroxide, hydrogen peroxide reacts with the chromogenic agent to produce red products, with a strong absorption peak at 505 nm wavelength. In a certain concentration range, the absorbance is linear with glucose concentration. Therefore, by measuring absorbance at 505 nm, glucose production can be calculated, and thus the sucrase activity can be calculated.

It is suitable for detecting the activity of sucrase in animal tissue samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissue samples