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Analysis of Mitochondrial Complex II Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1379-LGZ)
Introduction
Sensitivity: 0.54 U/L
Detection range: 0.54-17.66 U/L
Precision: inter-lot difference of 5%, intra-lot difference of 4%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 590-610 nm
Principle
The catalytic product of complex Ⅱ, reducing coenzyme Q, can further reduce 2, 6-dichloroindoxol. 2, 6-dichloroindoxol has a characteristic absorption peak at 600 nm. The enzyme activity can be calculated by measuring the reduction rate of 2, 6-dichloroindoxol.
Applications
It is suitable to detect the activity of mitochondrial respiratory chain complex II in animal tissue and cell samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: animal tissue and cell samples
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