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Analysis of Polyphenol Oxidase (PPO) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1362-LGZ)
Introduction
Precision: inter-lot difference of 9.800000000000001%, intra-lot difference of 4.6%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 410 nm
Principle
Polyphenol oxidase (PPO) can catalyze the oxidation of phenolic compounds to quinones. The latter has characteristic light absorption at 410 nm. The activity of PPO enzyme can be calculated by comparing with the increase of absorbance value.
Applications
It is suitable for detecting the activity of polyphenol oxidase (PPO) in plant tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: plant tissue samples
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