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Analysis of Glutathione Reductase (GR) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1324-LGZ)
Introduction
Sensitivity: 6.2 U/L
Detection range: 6.2-320 U/L
Precision: inter-lot difference of 2.5%, intra-lot difference of 2.1%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 340 nm
Principle
The catalytic oxidation of glutathione (GSSG) was catalyzed by NADPH to supply hydrogen and reduced to GSH. The activity of GR could be calculated by detecting the change of NADPH at 340 nm.
Applications
It was used to detect GR activity in serum, plasma, tissue and cell samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: Serum, plasma, tissue, cell
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