Analysis of Creatinine (Cr) by Enzyme-labeled Instrument (CAT#: STEM-MB-1406-LGZ)

Creatinine is catalyzed by creatinase to produce creatine, creatine is catalyzed by creatinase to produce creatine and urea, and creatine is catalyzed by sarcosine oxidase to produce glycine, formaldehyde and hydrogen peroxide. Hydrogen peroxide reacts with color developing agent under the catalysis of peroxidase to produce pink compound. The creatinine content can be determined by measuring OD value change at 515 nm wavelength.

Applicable to the detection of creatinine in serum, plasma, urine and other samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, urine and other samples