Analysis of β-Amylase Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1415-LGZ)

The reducing sugar reacted with 3, 5-dinitrosalicylic acid under the condition of heating to produce brown red substance. The β-amylase was passivated by taking advantage of the heat-resistant property of β-amylase. The total enzyme activity and α-amylase activity were measured, and the β-amylase activity was measured indirectly.

It was used to detect β-amylase activity in plant tissue samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: plant tissue samples